Sex differences and left-right asymmetries in expression of insulin and insulin-like growth factor-1 receptors in developing rat hippocampus J Hami, A Sadr-Nabavi, M Sankian et al. - Brain Structure and ..., 2012 - Springer
... Primary antibody incubation was performed at 4°C overnight using the following antibodies: rabbit anti-IGF-1R (antibody) (1:500, Signalway Antibody SAB, Pearland, USA), rabbit anti-InsR (antibody) (1:500, Abnova, Taipei, Taiwan), and rabbit anti-B-Actin (anti- body) (1:1,000 ...

Differential apoptosis gene expressions of rhabdomyosarcoma cells in response to enterovirus 71 infection W Shi, X Li, X Hou, H Peng, Q Jiang, M Shi et al. - BMC infectious ..., 2012 - biomedcentral.com
... Anti-caspase-8, -3, AKT2, JNK1/2, c-Jun and NF-kB p65 rabbit polyclonal antibodies were purchased from Signalway Antibody (Pearland, TX, USA). Rabbit polyclonal phospho-specific antibodies, including p-JNK1/2, p-AKT2 ...

ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promotes the Warburg effect W Yang, Y Zheng, Y Xia, H Ji, X Chen, F Guo et al. - Nature cell ..., 2012 - nature.com
... out with antibodies against the indicated proteins. (a) In vitro kinase assays were carried out with purified active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant. WB, Western blot. (b) Flag-PKM1 or Flag-PKM2 was immunoprecipitated (IP) with anti-Flag antibody from U87 ...

The anti-inflammatory mechanism of heme oxygenase-1 induced by hemin in primary rat alveolar macrophages C Hualin, X Wenli, L Dapeng, L Xijing, P Xiuhua… - Inflammation, 2012 - Springer
... American); HO-1 rabbit polyclonal primary antibody (1:1,000 dilution; Millipore, USA); phosphorylated p38 rabbit polyclonal primary antibody (1:750 dilution; Signalway Antibody Co., Ltd., USA); AP-conjugated goat antirabbit or antimouse secondary antibodies (Santa Cruz ...

Prognostic impact of Raf-1 and p-Raf-1 expressions for poor survival rate in non-small cell lung cancer ZX Qiu, L Wang, J Han, D Liu, W Huang, K Altaf et al. - Cancer ..., 2012 - Wiley Online Library
... Primary antibodies were: Rabbit anti-Raf-1 (Signalway Antibody, MD, USA), Rabbit anti-Raf-1 (phospho-ser259) (Signalway Antibody, College Park, MD, USA), Rabbit anti-phospho P70S6kinase (Cell Signaling, Shanghai, China), Mouse anti-GAPDH Ki67 (Thermo Fisher ...

Activation of Akt/FoxO and inactivation of MEK/ERK pathways contribute to induction of neuroprotection against transient global cerebral ischemia by delayed hypoxic ... L Zhan, D Li, D Liang, B Wu, P Zhu, Y Wang, W Sun et al. - , 2012 - Elsevier
... β-actin (1:1000). All these antibodies were purchased from Cell Signaling Technology, except for phospho-FKHR, phospho-FKHRL1 and phospho-AFX from Signalway Antibody. Densitometric analysis for the quantification ... Cited by 21 Related articles All 5 versions Cite Save

The Wnt signaling pathway effector TCF7L2 is upregulated by insulin and represses hepatic gluconeogenesis W Ip, W Shao, YA Chiang, T Jin - American Journal of ..., 2012 - Am Physiological Soc
... Antibodies for phosphorylated β-cat (Ser 675 ), β-cat, β-actin, cyclin D1, Akt, and phosphorylated ERK1 (Tyr 204 ) were purchased from Santa Cruz Biotechnology. The antibody for phosphorylated Akt (Ser 473 ) was the product of Signalway Antibody and for PEPCK was that of ...

Nuclear translocation of β-catenin is essential for glioma cell survival Z Shi, X Qian, L Li, J Zhang, S Zhu, J Zhu et al. - Journal of Neuroimmune ..., 2012 - Springer
... The membranes were incubated in the above-described primary antibodies, fol- lowed by incubation with HRP-conjugated secondary antibody (Zhongshan Bio ... The membrane was stripped and reprobed with Histone H2A.X (Signalway Antibody; 1:1000 dilution), β-actin ...

Prevention of acute kidney injury in a rodent model of cirrhosis following selective gut decontamination is associated with reduced renal TLR4 expression N Shah, D Dhar, FEZ Mohammed, A Habtesion et al. - Journal of ..., 2012 - Elsevier
... The proteins transferred to the membrane were probed using primary antibodies TLR4 (rabbit polyclonal to TLR4; Abbiotec, UK, 1:1000 dilution for 16 h at 4 °C overnight) and NFκBp65 (rabbit polyclonal to NFκBp65 [ab-536]; Signalway antibody, UK, 1:500 dilution for 16 h at 4 ...

Clinical significance of immunohistochemical expression of insulin-like growth factor-1 receptor and matrix metalloproteinase-7 in resected non-small cell lung ... T Yamamoto, T Oshima et al. - Experimental and ..., 2012 - spandidos-publications.com
... After quenching the endogenous peroxidase activity with 3% H2O2 for 5 min, the sections were incubated for 60 min at room temperature with the primary antibody diluted at 1:100 for IGF-1R (a rabbit polyclonal antibody, clone 1161; Signalway Antibody, Pearland, TX, USA). ...