Pre-treatment of human umbilical cord-derived mesenchymal stem cells with interleukin-6 abolishes their growth-promoting effect on gastric cancer cells M Wang, J Cai, F Huang, M Zhu et al. - International ..., 2015 - spandidos-publications.com
... Following incubation with the secondary antibodies (Cat. no. 13012-2; Signalway Antibody Co., Ltd.), the signal was visualized using HRP substrate (Millipore, Billerica, MA, USA) and analyzed using MD Image Quant Software. GAPDH was used as the loading control. ...

Impairment of Rat Spatial Learning and Memory in a New Model of Cold Water-Induced Chronic Hypothermia: Implication for Alzheimer's Disease MM Ahmadian-Attari, L Dargahi, M Mosaddegh et al. - Neurotoxicity ..., 2015 - Springer
... Purified rabbit polyclonal anti-tau antibody pS396 (phosphor-Ser396; 1:800 v/v, Signalway Antibody), rabbit polyclonal anti-total tau antibody (1:800 v/v, Signalway Antibody), Bax rabbit polyclonal antibody (1:1000 v/v; cell signaling), Bcl2 rabbit polyclonal antibody (1:1000 v/v ...

Acetylcholine Inhibits Tumor Necrosis Factor α Activated Endoplasmic Reticulum Apoptotic Pathway via EGFR-PI3K Signaling in Cardiomyocytes Y Miao, XY Bi, M Zhao, HK Jiang, JJ Liu et al. - Journal of cellular ..., 2015 - Wiley Online Library
... antibodies: GAPDH Mouse Monoclonal Antibody (Sinopept, Beijing, China), M AChR M2 pAb, EGFR pAb, CHOP pAb (Bioworld Technology, MN), caspase-3 antibody, GRP78 antibody, PERK antibody (Cell Signaling Technology, Beverly, MA), Akt (Signalway Antibody, ...

MicroRNA-145 inhibits lung cancer cell metastasis DJ Ling, ZS Chen, YD Zhang et al. - Molecular ..., 2015 - spandidos-publications.com
... The following antibodies: Rabbit polyclonal Oct4 (sc-9081), mouse monoclonal Wnt3a (sc ... Rabbit polyclonal vimentin antibody (ab92547) was obtained from Abcam (Cambridge, MA, USA ... rabbit polyclonal pan-cytokeratin (SAB, 23032) were obtained from Signalway Antibody Inc ...

Carbocysteine restores steroid sensitivity by targeting histone deacetylase 2 in a thiol/GSH-dependent manner Y Song, HZ Lu, JR Xu, XL Wang, W Zhou et al. - Pharmacological ..., 2015 - Elsevier
... were lastly re-probed with an anti-Histone H2A.X antibody (Signalway Antibody, USA) as loading ... Anti-HDAC2 primary antibody (mouse anti-human; Cell Signaling Technology, USA) was incubated overnight, followed by incubation with secondary antibodies (DyLight 555 ...

Protection of Quercetin against Triptolide-induced apoptosis by suppressing oxidative stress in rat Leydig cells J Hu, Q Yu, F Zhao, J Ji, Z Jiang, X Chen, P Gao et al. - Chemico-biological ..., 2015 - Elsevier
... Antibodies to caspase-9, caspase-3 and bcl-2 were purchased from Cell Signaling Technology (CST, MA, USA). Anti-Cytochrome c antibody was purchased from GeneTex (GeneTex, CA, USA). Anti-Bax antibody was purchased from Signalway Antibody (SAB, CA, USA). 2.2. ...

Lipoxin A4 methyl ester alleviates vascular cognition impairment by regulating the expression of proteins related to autophagy and ER stress in the rat hippocampus Y Jia, W Jin, Y Xiao, Y Dong, T Wang, M Fan et al. - Cellular and Molecular ..., 2015 - degruyter.com
... Membranes were blocked with 5% non-fat milk in Tris-buffered saline Tween-20 (TBST) for 2 h at room temperature, then incubated with primary antibodies against the following proteins at 4°C overnight with gentle shaking: GRP78 (1:1,000, Signalway Antibody), CHOP (1:1,000 ...

The role of cytoplasmic p57 in invasion of hepatocellular carcinoma H Guo, Y Li, T Tian, L Han, Z Ruan, X Liang et al. - BMC ..., 2015 - biomedcentral.com
... The membranes were blocked and subsequently incubated with the following primary antibodies: anti-p57 antibody (1:500), anti-phospho-cofilin (Ser3) antibody (1:300, 11139, Signalway Antibody, Pearland, TX, USA), anti-β-actin antibody (1:1000, sc-130301, Santa Cruz) and ...

Lithium chloride administration prevents spatial learning and memory impairment in repeated cerebral ischemia-reperfusion mice by depressing apoptosis and ... M Fan, W Jin, H Zhao, Y Xiao, Y Jia, Y Yin et al. - Behavioural Brain ..., 2015 - Elsevier
... Antibody, Pearland, TX, USA, 1:500), anti-CREB (Signalway Antibody, Pearland, TX ... signaling technology, Boston, MA, USA, 1:1000), or GAPDH antibody (Bioworld Technology ... were incubated with a horseradish peroxidase conjugated secondary antibodies (Proteintech Group ...

Arsenic Trioxide Activate Transcription of Heme Oxygenase-1 by Promoting Nuclear Translocation of NFE2L2 Z Yue, L Zhong, Y Mou, X Wang, H Zhang et al. - journal of medical ..., 2015 - ncbi.nlm.nih.gov
... Nuclear protein was extracted by a nuclear protein extraction kit from Signalway Antibody (Maryland, USA), following the manufacturer's instruction. ... were incubated with primary (rabbit anti-human HO-1 and rabbit anti-human NFE2L2 polyclonal antibodies and mouse anti ...