Metabolic inflammation exacerbates dopaminergic neuronal degeneration in response to acute MPTP challenge in type 2 diabetes mice L Wang, YQ Zhai, LL Xu, C Qiao, XL Sun, JH Ding et al. - Experimental ..., 2014 - Elsevier
... The membranes were incubated with various primary antibodies overnight at 4 °C. The primary antibodies were rabbit anti-IRS-1 (21223, Signalway Antibody, Nanjing, China), rabbit anti-phospho-IRS-1 ser636 (11230, Signalway Antibody, Nanjing, China), rabbit anti-Akt ...

Small molecule MIRA-1 induces in vitro and in vivo anti-myeloma activity and synergizes with current anti-myeloma agents MN Saha, Y Chen, MH Chen, G Chen… - British journal of ..., 2014 - nature.com
... Immunoblotting was performed using specific antibodies: anti-p53 (DO-7) (Sigma-Aldrich); Mcl-1, caspase-3, and PARP (Cell Signaling Technologies, Beverly, MA, USA); Puma, Bax, and c-Myc (Signalway Antibody, College Park, MD, USA); XBP1 and p-PERK (Thr 981) (Santa ...

The use of hollow mesoporous silica nanospheres to encapsulate bortezomib and improve efficacy for non-small cell lung cancer therapy J Shen, G Song, M An, X Li, N Wu, K Ruan, J Hu, R Hu - Biomaterials, 2014 - Elsevier
... The following antibodies were used: rabbit anti-Pi-Akt antibody (Signalway Antibody), rabbit anti-Akt antibody (Signalway Antibody), mouse anti-Cyclin B antibody (Santa Cruz), rabbit anti-Caspase 3 antibody (Signalway Antibody), mouse anti-actin antibody (Sigma), rabbit anti ...

Necroptosis inhibitor necrostatin-1 promotes cell protection and physiological function in traumatic spinal cord injury Y Wang, H Wang, Y Tao, S Zhang, J Wang, X Feng - Neuroscience, 2014 - Elsevier
... membrane was incubated in primary antibodies diluted in 5% fat-free milk overnight at 4 °C. The antibodies included anti-cleaved Caspase 3 (Rabbit Polyclonal IgG, 1:200. Millipore, Billerica, MA, USA), anti-Bcl2 (Rabbit Polyconal IgG, 1:800. Signalway Antibody, College Park ...

Aerobic exercise inhibits sympathetic nerve sprouting and restores β-adrenergic receptor balance in rats with myocardial infarction T Chen, MX Cai, YY Li, ZX He, XC Shi, W Song… - 2014 - dx.plos.org
... The membrane was incubated with the following diluted primary antibodies: TH (1:500, Millipore), GAP43 (1:1000, Millipore), NGF (1:500, Signalway Antibody), β1-AR (1:2000, Bioworld), β2-AR (1:1000, Signalway Antibody), ...

Curcumin suppresses cell proliferation through inhibition of the Wnt/β-catenin signaling pathway in medulloblastoma M He, Y Li, L Zhang, L Li, Y Shen, L Lin et al. - Oncology ..., 2014 - spandidos-publications.com
... rabbit anti-human phospho-β-catenin (Ser37) antibody (11219-2; Signalway Antibody, College Park ... Rabbit anti-human β-actin polyclonal antibody (CW0097; CWBIO, Beijing, China) was used ... rabbit IgG (ZF-0311; ZSGB-BIO, Beijing, China) were used as secondary antibodies. ...

Cryptotanshinone inhibits lung tumorigenesis and induces apoptosis in cancer cells in vitro and in vivo L Chen, HJ Wang, W Xie, Y Yao et al. - Molecular ..., 2014 - spandidos-publications.com
... electrophoresis and transfer, the polyvinylidene fluoride (PVDF) membrane (Invitrogen, USA) was blocked in 5% non-fat milk for 1 h, incubated overnight at 4°C with B-cell lymphoma 2 (Bcl2) rabbit polyconal immunoglobulin G (IgG) (1:1000; Signalway Antibody LLD, College ...

The effects of cannabidiol and its synergism with bortezomib in multiple myeloma cell lines. A role for transient receptor potential vanilloid type-2 MB Morelli, M Offidani, F Alesiani et al. - Journal of Cancer, 2014 - Wiley Online Library
... The following mouse monoclonal antibodies (Abs) were used: phycoerythrin [PE]-conjugated anti-human CD138 (20 μl/sample), PE-conjugated ... 1:1,000) and rabbit anti-ERK (1:1,000) (Cell Signalling Technology); rabbit anti-AKT (1:1,000) (Signalway Antibody, Pearland, TX ...

Quercetin regulates the sestrin 2-AMPK-p38 MAPK signaling pathway and induces apoptosis by increasing the generation of intracellular ROS in a p53- ... GT Kim, SH Lee, JI Kim, YM Kim - journal of molecular ..., 2014 - spandidos-publications.com
... Specific antibodies that recognized phosphorylated (p-)AMPKα1, AMPKα1, Bax, caspase-3, cytochrome c and β-actin are obtained from Cell Signaling Technology (Beverly, MA, USA) and ... p-p38 MAPK was purchased from Signalway Antibody LLC (College Park, MD, USA). ...

Fermentation of soy milk via Lactobacillus plantarum improves dysregulated lipid metabolism in rats on a high cholesterol diet Y Kim, S Yoon, SB Lee, HW Han, H Oh, WJ Lee et al. - PloS one, 2014 - dx.plos.org
... anti-p-AMP-activated protein kinase (AMPK) and anti-p-Acetyl CoA carboxylase (ACC) antibodies from Cell Signaling Technology (Beverly, MA, USA), anti-LDLR antibody from BioVision (Milpitas, CA, USA), and anti-GAPDH antibody from Signalway Antibody (College Park, MD ...