A simple and fast method of solid phase extraction (SPE) disk for the determination of trace acrylamide was developed. Using this new technique along with high performance liquid chromatography (HPLC) and ultraviolet spectroscopy (UV) detection, the feasibility of SPE disk method for rapid enrichment was demonstrated in the determination of trace acrylamide in 500 mL tap water. Active carbon was chosen as the adsorbent to be incorporated into the SPE disk as it gave the best adsorption efficiency.

Another material under investigation for preparing plasma samples is diatomaceous earth or diatomite-like substrate. The porous and channel structures of diatomite offer excellent dispersive properties to trap large molecules such as phospholipids. When a plasma sample passes this specially treated diatomite material, it will spread and penetrate into the pores, to form an aqueous film on the surface of the packing. When a water-immiscible liquid is passing through the diatomite, LLE occurs on the solid support. The procedure is called supported liquid extraction (SLE).

Bonna-Agela, a chromatography specialty company, has developed many HPLC methods for Pharmacopoeia applications. Many of these methods using Bonna-Agela columns have been acknowledged by USP-PQRI column database for better solutions of HPLC applications. Detailed information and methods of selection of your HPLC columns can be found at here.

* Fengyan Jia, Wenwen Wang, Juan Wang, Jungang Yin, Yongming Liu and Zhenbo Liu (2012) New strategy to enhance the extraction efficiency of pyrethroid pesticides in fish samples using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method, Anal. Methods, 4, 449-453; DOI: 10.1039/C2AY05681J

Methods:

Determination melamine in fish, milk and eggs by MAS-HPLC

Instrument: L6-1 series HPLC

Sample preparation method kit for melamine determination, including HCl 0.1mol/L, 6% sulfosalicylic acid, Venusil SCX-M and its guard cartridge; syringe filter; melamine standard (>99%);

Reagents: acetonitrile (chromatographic grade), potassium dihydrogen phosphate (analytical grade), ultrapure water.

Releated product from Bonna-Agela

More methods:

Determination of β-Estradiol Residues in Fish/Shellfish Muscle by Gas Chromatography-Mass Spectrometry