BAD overexpression inhibits cell growth and induces apoptosis via mitochondrial-dependent pathway in non-small cell lung cancer L Jiang, M Luo, D Liu, B Chen, W Zhang, L Mai et al. - Cancer Cell ..., 2013 - biomedcentral.com
... The membranes were incubated overnight at 4°C with anti- BAD monoclonal antibody (#9292, 1;1000, Cell Signaling Technology, Beverly, USA), Bcl-xl (#24247, 1:1000, Signalway Antibody, Pearland, USA), Bcl-2 (#24246, 1:1000, Signalway Antibody), Bax (#24250, 1:1000 ...

Nuclear PKM2 regulates the Warburg effect W Yang, Z Lu - Cell cycle, 2013 - Taylor & Francis ... EGF and mouse monoclonal antibody for Flag were pur- chased from Sigma. ... with use of an RNA
High-Purity Total RNA Rapid Extraction Kit (Signalway Biotechnol- ogy). ... PCR analysis was performed using 2' SIBR Real-Time PCR Premixture (Signal- way Biotechnology) under ...

H. pylori infection-induced MSC differentiation into CAFs promotes epithelial-mesenchymal transition in gastric epithelial cells Q Zhang, M Wang, F Huang et al. - International ..., 2013 - spandidos-publications.com
... The sources and dilution factors of the primary antibodies were as follows: rabbit polyclonal anti-FAP (1:500; Abcam, USA), anti-vimentin (1:2,000; Santa Cruz Biotechnology, Inc.), anti-N-cadherin (1:400; SAB; Signalway Antibody Co., Ltd., MD, USA), anti-E-cadherin (1:500 ...

MicroRNA-19a/b regulates multidrug resistance in human gastric cancer cells by targeting PTEN F Wang, T Li, B Zhang, H Li, Q Wu, L Yang et al. - Biochemical and ..., 2013 - Elsevier
... was probed with primary monoclonal antibodies specific to Bcl-2 (1:200, Santa Cruz Biotechnology, CA), Bax (1:100, ZSGB), Caspase-3 (1:200, ZSGB), P-gp (1:200, Santa Cruz Biotechnology), PTEN (1:500, Cell Signaling Technology), AKT (1:500, Signalway Antibody) or ...

Epidermal growth factor (EGF)-enhanced vascular cell adhesion molecule-1 (VCAM-1) expression promotes macrophage and glioblastoma cell interaction and tumor ... Y Zheng, W Yang, K Aldape, J He, Z Lu - Journal of Biological Chemistry, 2013 - ASBMB
... Tyr-1172 polyclonal antibody (11220) was obtained from Signalway Biotechnology (Pearland, TX) and Signalway Antibody (College Park ... Anti-tubulin monoclonal antibody was from Sigma. Rabbit polyclonal antibodies for PKCε (sc-214) and IκBα (sc-371) were purchased from ...

Cellular mechanism by which estradiol protects female ovariectomized mice from high-fat diet-induced hepatic and muscle insulin resistance JPG Camporez, FR Jornayvaz, HY Lee et al. - ..., 2013 - press.endocrine.org
... nonspecific sites with 5% nonfat dry milk Tris-buffered saline and Tween 20 (10mM Tris, 100mM NaCl, and 0.1% Tween 20) solution, membranes were incubated overnight at 4°C with the following primary antibodies: phopho-AKT2 ser473 (Signalway Antibody, Pearland, Texas ...

Magnesium deficiency promotes secretion of high-mobility group box 1 protein from lipopolysaccharide-activated macrophages in vitro Z Liu, Y Chang, J Zhang, X Huang, J Jiang, S Li et al. - Journal of Surgical ..., 2013 - Elsevier
... Cruz Biotechnology, Santa Cruz, CA), or anti-histone H3.1 antibody (1:1000; SAB Signalway Antibody, Pearland, TX) for 1 h at room temperature. After three washes with PBS-T, the membranes were incubated with horseradish peroxidase-linked secondary antibodies (1:1000 ...

Cleavage of GSK-3β by calpain counteracts the inhibitory effect of Ser9 phosphorylation on GSK-3β activity induced by H2O2 Y Feng, Y Xia, G Yu, X Shu, H Ge, K Zeng et al. - Journal of ..., 2013 - Wiley Online Library
... phospho-AKT (Ser473), and Fyn were obtained from Cell Signaling Technology Inc (Beverly, MA, USA); rabbit polyclonal antibody GSK-3β (Ab-216, #21301) was purchased from Signalway Antibody LLC (College Park, MD, USA); rabbit polyclonal antibodies including phospho ...

PIK3R1 underexpression is an independent prognostic marker in breast cancer M Cizkova, S Vacher, D Meseure, M Trassard et al. - BMC ..., 2013 - biomedcentral.com
... rabbit polyclonal antibody directed against human p85 protein (Signalway Antibody, Baltimore, Maryland). The localization and intensity of staining were assessed by two independent pathologists blinded to real-time RT-PCR results. Both antibodies were used ...

Aberrant hypermethylation and reduced expression of disabled-2 promote the development of lung cancers XM Xie, ZY Zhang, LH Yang et al. - International ..., 2013 - spandidos-publications.com
... anti-Dab2 (1:500, Santa Cruz Biotechnology Inc., Santa Cruz, CA, USA), anti-β-catenin (1:500, BD Transduction Laboratories, KY, USA), anti-β-actin (1:500, BD Transduction Laboratories), and anti-histone 3.1 (1:400, Signalway Antibody, College Park, MD, USA) antibodies. ...