Rb1 postconditioning attenuates liver warm ischemia–reperfusion injury through ROS-NO-HIF pathway Y Guo, T Yang, J Lu, S Li, L Wan, D Long, Q Li, L Feng et al. - Life sciences, 2011 - Elsevier
... HIF-1alpha (diluted at 1:500, AbCam, Canbridge, UK); iNOS (diluted at 1:500, AbCam, Canbridge, UK); p-Akt (diluted at 1:500, Signalway Antibody); rabbit polyclonal antibody against Akt (diluted at 1:500, Signalway Antibody); followed by secondary antibodies (diluted at 1 ...

Ischemic postconditioning attenuates liver warm ischemia-reperfusion injury through Akt-eNOS-NO-HIF pathway JY Guo, T Yang, XG Sun, NY Zhou, FS Li, D Long et al. - J Biomed, 2011 - biomedcentral.com
... for 2 h at 37 °C with a mouse monoclonal anti- body against HIF-1a (diluted 1:500, AbCam, Canbridge, UK); p-Akt (diluted 1:500, Signalway Antibody); rabbit polyclonal antibody against Akt (diluted 1:500, Signal- way Antibody); followed by a secondary antibodies (diluted 1 ...

Activation of mammalian target of rapamycin pathway confers adverse outcome in nonsmall cell lung carcinoma D Liu, Y Huang, B Chen, J Zeng, N Guo, S Zhang et al. - Cancer, 2011 - Wiley Online Library
... Antibodies used were as follows: mTOR (7C10, 1:50 dilution, #2983, Cell Signaling Technology, Beverly, Mass), phosphorylated (p-)mTOR (Ser2448, 49F9, 1:100 dilution, #2976, Cell Signaling Technology), PDK1 (1:300 dilution, #21,005, Signalway Antibody, Pearland, Tex), p ...

The effects of artesunate on the expression of EGFR and ABCG2 in A549 human lung cancer cells and a xenograft model H Ma, Q Yao, AM Zhang, S Lin, XX Wang, L Wu et al. - Molecules, 2011 - mdpi.com
... (Hong Kong, China). Rabbit polyclonal antibodies to Akt (Ab-473) and p-Akt (Phospho-Ser473) were purchased from Signalway Antibody Co., Ltd (Pearland, TX, USA), and goat anti-rabbit IgG (peroxidase conjugate) and goat anti-rat IgG (peroxidase conjugate) were purchased ...

Downregulation and translocation of nuclear ING4 is correlated with tumorigenesis and progression of head and neck squamous cell carcinoma X Li, K Kikuchi, Y Zheng, A Noguchi, H Takahashi et al. - Oral oncology, 2011 - Elsevier
... Membranes were blocked in 5% skim milk in TBS-T (10 mM Tris–HCl, 150 mM NaCl, 0.1% Tween 20) for 1 h, then was incubated with antibodies against ING4 (1:200, Proteintech Group, Inc, Chicago, IL, USA), H 2 AX (1:500, Signalway Antibody, TX) and β-actin (1:500 ...

Canonical notch pathway protects hepatocytes from ischemia/reperfusion injury in mice by repressing reactive oxygen species production through JAK2/ ... HC Yu, HY Qin, F He, L Wang, W Fu, D Liu et al. - 2011 - Wiley Online Library
... Western blot analysis was performed routinely, with primary antibodies including anti-Hes5, anti-MnSOD (Santa Cruz Biotechnology), anti-phospho-STAT3, anti-STAT3, anti-Akt, anti–phospho-Akt (Signalway Antibody, Pearland, TX), ...

Asperosaponin VI, A Saponin Component from Dipsacus asper Wall, induces Osteoblast Differentiation through Bone Morphogenetic Protein - 2/p38 and Extracellular ... Y Niu, Y Li, H Huang, X Kong, R Zhang et al. - Phytotherapy ..., 2011 - Wiley Online Library
... Primary antibodies against the following targets were used: BMP-2, ERK1/2, phospho-ERK1/ 2 (Signalway Antibody, TX, USA); p38, phospho-p38, BrDU (Biosynthesis Biotechnology, Beijing, China) and ...

Adaptive gene regulation of pyruvate dehydrogenase kinase isoenzyme 4 in hepatotoxic chemical-induced liver injury and its stimulatory potential for DNA repair and ... M Dateki, M Kunitomo, K Yoshioka et al. - Journal of Receptors, 2011 - Taylor & Francis
... The dilution ratio of the first antibodies to each factor was as follows: PDK4 (Abgent, USA), x500; cell division control protein 2 (CDC2; phospho Thr161; Signalway Antibody, USA), x1000; CDC2 (phospho Tyr15; Signalway Antibody, USA), x1000; ...

Downregulation of p57 accelerates the growth and invasion of hepatocellular carcinoma H Guo, Y Lv, T Tian, TH Hu, WJ Wang, X Sui et al. - , 2011 - Oxford Univ Press
... The membranes were blocked and subsequently incubated with the following primary antibodies: anti-p57 antibody (1:500 ... Thr508) antibody (1:250, PAB10997; Abnova, Taiwan, China), anti-phospho-cofilin (Ser-3) antibody (1:300, 11139, Signalway Antibody, Pearland, TX ...

Triptolide ameliorates Crohn's colitis is associated with inhibition of TLRs/NF-kB signaling pathway C Yu, T Shan, A Feng, Y Li, W Zhu, Y Xie, N Li, J Li - Fitoterapia, 2011 - Elsevier
... antibodies at 4 °C. Antibodies against TLR2, TLR4, Myeloid differentiation factor 88 (MyD88), NF-kB p65 (all from Santa Cruz Biotechnology, Santa Cruz, CA, USA) and anti-Histone H3.1 (from Signalway Antibody Company, Pearland, TX) were all used at a 1:500 dilution. ...