Our typical turn-around time is about 2-3 weeks. The turn-around time may vary depending on the peptide length and difficulty.

All peptides will be shipped lyophilized in small microcentrifuge tubes (2 ml). Large amount of peptide may be aliquot into several tubes. For every single peptide, data sheets will be provided that contains the key characteristics, such as amino acid sequence, modification, purity, mass spectrum data and HPLC data.

All solubility information is reported on the Certificate of Analysis received with your peptide. Please read the instructions carefully before starting. Solubility may vary depending on the nature of the peptide. The most common dissolution process is 1 mg of peptide distilled in 1 ml of sterile Milli-Q water.

The solubility of peptides varies depending on its amino acid sequence and modifications. When we prepare it, the peptide is generally purified by HPLC using water and acetonitrile gradient. Here are some general tips for dissolving peptides:

• Sonification will increase the solubility of peptides.
• For basic peptides, 10% acetic acid in the solvent will help dissolve peptides.
• For acidic peptides, 10% ammonium bicarbonate will help dissolve peptides.
• For peptides with extremely low solubility in aqueous solutions, organic solvents should be used first (such as DMSO, isopropanol, methanol, acetonitrile). Once peptides are dissolved completely, water may be added slowly until the final concentration has been reached.

Solubility is often one of the major challenges for researchers who work with peptides. Each amino acid exhibits its own physical characteristics. Amino acids such as leucine, isoleucine, and valine are very hydrophobic. Other amino acids like lysine, histidine, and arginine are hydrophilic. Thus, different peptides will have different solubilities, based on the content of the peptides.

If storage of peptides in solution is absolutely unavoidable, use sterile buffers at pH 5-6 and store aliquots at -20⁰ C to prolong the storage life of peptides in solution.

It is not recommended to keep excess peptides in solution. The shelf life of peptides in solution is very limited, especially for sequences containing cysteine, methionine, tryptophan, asparagine, glutamine, and N-terminal glutamic acid. In general, aliquot the necessary amounts of peptide for a few days and relyophilize remaining portions for long term storage if necessary.