Features and Benefits

Carryover Contamination Prevention Minimized false positives caused by a carryover contamination through application of uracil DNA glycosylase system
User-friendly Simplified procedure by packaging each of the tubes with sufficient amounts of reactants required for one run, allowing anyone to perform PCR tests simply by adding template DNA and primers
Stability Stabilizer included in the PCR reaction mixture to keep stable enzymatic activities for up to 2 years if stored at -20 ° C.
Reproducibility Minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variables by a mass production under ISO 9001 quality system

Application

Conventional PCR
Primer extension
TA cloning
Gene sequencing
Molecular diagnosis

Experimental data

Figure. Comparison of sensitivity between AccuPower^® PCR PreMix and AccuPower^® PCR PreMix (with UDG)
Sensitivity test was operated using serial diluted human genomic DNA. Reaction mixture was incubated at 37°C for 2min followed by 95°C for 5min, 30 cycles of 20 sec at 95°C, 20 sec at 55°C, 30 sec at 72°C. AccuPower^® PCR PreMix (with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.

The amount of DNA used to test is represented below.

Lane 1; 10 ng of Human genomic DNA
Lane 2; 1 ng of Human genomic DNA
Lane 3; 100 pg of Human genomic DNA
Lane 4; 10 pg of Human genomic DNA
M; 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)

Technical / Specifications

▶ Specifications

Enzyme	Top DNA polymerase
5' to 3' exonuclease activity	No
3' to 5' exonuclease activity	No
Terminal transferase activity	Yes
Fragment Size	Up to 10 kb

▶ Experimental data

Figure 1.Comparison of amplification quality using PCR products (not including uracil base)
between AccuPower^® PCR PreMix and AccuPower^® PCR PreMix (with UDG).

Amplification quality test was operated using serial diluted PCR products not including uracil base.
AccuPower^® PCR PreMix was also tested for negative control. Reaction mixture was incubated at 37°C for 2 min, followed by 95°C for 5min, 30 cycles of 20 sec at 95°C, 20 sec at 55°C, 30 sec at 72°C.
AccuPower^® PCR PreMix (with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.

The copy number of PCR products (copy) used to test is represented below.

Lane 1; 10¹¹copy
Lane 2; 10¹⁰copy
Lane 3; 10⁹copy
Lane 4; 10⁸copy
Lane 5; 10⁷copy
Lane 6; 10⁶copy
Lane 7; 10⁵copy
Lane 8; 10⁴copy
Lane 9; 10³copy
Lane 10; 10²copy
Lane N; No template control
M; 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)

Figure 2. Efficiency of uracil DNA glycosylase using PCR products (including uracil base)
Efficiency test of uracil DNA glycosylase was operated using serial diluted PCR products including uracil base.
AccuPower^® PCR PreMix was also tested for negative control. Reaction mixture was incubated at 37°C for 2 min, followed by 95°C for 5 min, 30 cycles of 20 sec at 95°C, 20 sec at 55°C, 30 sec at 72°C. AccuPower^® PCR PreMix (with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.

The copy number of PCR products used to test is represented below.