Features and Benefits
- Stability Addition of stabilizing agents allows delivery at room temperature and enhanced stability compared to solution-type products
- User-friendly Amounts of reactants required for one PCR cycle included in each of the PCR tubes, allowing anyone to perform PCR tests simply by adding template DNA and primers
- Sensitivity Excellent sensitivity and amplification efficiency even with small amounts of DNA
- Reproducibility Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under same conditions and variables
Application
- Conventional PCR
- Primer extension
- TA cloning
- Gene sequencing
Experimental data
Figure. Comparison of heat-stability of AccuPower® PCR PreMix
AccuPower® PCR PreMix is incubated at 95℃ with various time.
Kit: AccuPower® PCR PreMix (Batch-1/2)
A; 30 min, B; 60 min, C; 90 min
Lane 1; Human genomic DNA 10 ng
Lane 2; Human genomic DNA 1 ng
Lane 3; Human genomic DNA 100 pg
Lane 4; Human genomic DNA 10 pg
M ;100 bp plus DNA ladder (Bioneer Cat. No: D-1035)
Technical / Specifications
▶ Specifications
|
Enzyme |
Top DNA polymerase |
|
5' to 3' exonuclease activity |
No |
|
3' to 5' exonuclease activity |
No |
|
3' – A overhang |
Yes |
|
Fragment Size |
Up to 10 kb |
Ordering Information
| Cat. No. | Description | Option | Price (AUD) |
|---|---|---|---|
| K-2012 | AccuPower ® PCR PreMix & Master Mix | PreMix type | 0.2 ml thin-wall 8-strip tubes with attached cap | 96 tubes | $73.85 |
| K-2036 | AccuPower ® PCR PreMix & Master Mix | PreMix type | 0.2 ml thin-wall 8-strip tubes with attached cap | 96 tubes | $70.00 |
| K-2013 | AccuPower ® PCR PreMix & Master Mix | PreMix type | 0.2 ml thin-wall 8-strip tubes with attached cap | 96 tubes | $110.77 |
| K-2016 | AccuPower ® PCR PreMix & Master Mix | PreMix type | 0.2 ml thin-wall 8-strip tubes with attached cap | 480 tubes | $276.92 |
| K-2037 | AccuPower ® PCR PreMix & Master Mix | PreMix type | 0.2 ml thin-wall 8-strip tubes with attached cap | 480 tubes | $276.92 |
| K-2017 | AccuPower ® PCR PreMix & Master Mix | PreMix type | 0.2 ml thin-wall 8-strip tubes with attached cap | 480 tubes | $415.38 |
| K-2011 | AccuPower ® PCR PreMix & Master Mix | PreMix type | 0.5 ml thin wall microtube | 100 tubes | $130.77 |
| K-2260-1 | AccuPower ® PCR PreMix & Master Mix | PreMix type | thin-wall 96-well flat plate | $73.85 |
| K-2260-4 | AccuPower ® PCR PreMix & Master Mix | PreMix type | thin-wall 96-well flat plate | $81.54 |
| K-2260-2 | AccuPower ® PCR PreMix & Master Mix | PreMix type | thin-wall 96-well full-skirted plate | $73.85 |
| K-2260-5 | AccuPower ® PCR PreMix & Master Mix | PreMix type | thin-wall 96-well full-skirted plate | $81.54 |
| K-2260-3 | AccuPower ® PCR PreMix & Master Mix | PreMix type | thin-wall 96-well semi-skirted plate | $73.85 |
| K-2260-6 | AccuPower ® PCR PreMix & Master Mix | PreMix type | thin-wall 96-well semi-skirted plate | $81.54 |
| K-2080-1 | AccuPower ® PCR PreMix & Master Mix | PreMix type | thin-wall 384-well full-skirted plate | $300.00 |
| K-2080-2 | AccuPower ® PCR PreMix & Master Mix | PreMix type | thin-wall 384-well full-skirted plate | $315.38 |
| K-2080-3 | AccuPower ® PCR PreMix & Master Mix | PreMix type | thin-wall 384-well full-skirted plate | $338.46 |
| K-2018 | AccuPower ® PCR PreMix & Master Mix | MasterMix type | 1 ml of 2X Master mix solution | $65.38 |
| K-2018-1 | AccuPower ® PCR PreMix & Master Mix | MasterMix type | 10 ml of 2X Master mix solution | $438.46 |