Features and Benefits

Optimized buffer delivery Guaranteed stable response with Top optimized buffer for DNA polymerase
Efficiency & Sensitivity High efficiency and sensitivity for DNA amplification using PCR
Reproducibility Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system

Application

Real-time quantification of DNA and cDNA targets using dsDNA binding dye
Gene expression profiling
Microbial & viral pathogen detection

Experimental data

Figure. Sensitivity test of Top DNA polymerase using Lambda genomic DNA.
Each fragment was amplified from a template dilution series (10 ng to 10 fg DNA per reaction) using 1 U of each Top DNA polymerase.

Technical / Specifications

▶ Specifications

5' to 3' exonuclease activity	No
3' to 5' exonuclease activity	No
3' – A overhang	Yes
Fragment size	Up to 10 kb

▶ Components

• 10X reaction buffer with (or without) MgCl₂: Tris (pH 9.0), 15 mM MgCl₂, etc

• 1X dilution buffer: 50% glycerol containing 50 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, pH 8.0

• 10 mM dNTPs mix: 2.5 mM of each dNTP

Ordering Information

Cat. No.	Description	Option	Price (AUD)
E-3100	Top DNA Polymerase	500 U \| 10X reaction buffer with MgCl 2	$118.46
E-3100-2	Top DNA Polymerase	500 U	$84.62
E-3100-1	Top DNA Polymerase	500 U \| 10X reaction buffer, 20 mM MgCl 2	$118.46
E-3100-3	Top DNA Polymerase	500 U	$84.62
E-3101	Top DNA Polymerase	2,000 U \| 10X reaction buffer with MgCl 2	$415.38
E-3101-2	Top DNA Polymerase	2,000 U	$300.00
E-3101-1	Top DNA Polymerase	2,000 U \| 10X reaction buffer, 20 mM MgCl 2	$415.38
E-3101-3	Top DNA Polymerase	2,000 U	$300.00
D-3001	10 mM dNTP	1 ml (each 2.5 mM)	$52.31

Top DNA Polymerase