Features and Benefits
- Efficiency & Sensitivity High efficiency and sensitivity to the templates
- Optimized buffer delivery Stable PCR reaction by providing buffers optimized for the enzyme
- Reproducibility Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system
Application
Experimental data
Validation of enzymatic activities by using 1 unit of Taq DNA polymerase on: (A) human genomic DNA (B) Lambda DNA
Lane 1; 100 ng template DNA
Lane 2; 10 ng template DNA
Lane 3; 1 ng template DNA
Lane 4; 100 pg template DNA
Lane 5; 10 pg template DNA
Lane 6; 1 pg template DNA
Lane 7; 100 fg template DNA
Technical / Specifications
▶ Specifications
|
5' to 3' exonuclease activity |
Yes |
|
3' to 5' exonuclease activity |
No |
|
3' – A overhang |
Yes |
|
Fragment size |
Up to 10 kb |
▶ Components
• 10X reaction buffer with (or without) MgCl2: Tris-HCl, KCl, 15 mM MgCl2, pH 9.0
• Dilution buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol, pH 8.0
• 10 mM dNTPs mix: 2.5 mM of each dNTP
▶ Concentration
500 units (5 U/μl)
▶ Storage conditions
20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol pH 8.0
▶ Storage temperature
-20°C
▶ Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72℃.
▶ Concentration
500 units (5 U/μl)
▶ Storage conditions
20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol pH 8.0
▶ Storage temperature
-20°C
▶ Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72℃.
Ordering Information
| Cat. No. | Description | Option | Price (AUD) |
|---|---|---|---|
| E-2011 | Taq DNA Polymerase | 500 U | 10X Reaction Buffer with MgCl 2 | $118.46 |
| E-2011-2 | Taq DNA Polymerase | 500 U | $84.62 |
| E-2011-1 | Taq DNA Polymerase | 500 U | 10X Reaction Buffer, without 20 mM MgCl 2 | $118.46 |
| E-2011-3 | Taq DNA Polymerase | 500 U | $84.62 |
| E-2013 | Taq DNA Polymerase | 2,000 U | 10X Reaction Buffer with MgCl 2 | $415.38 |
| E-2013-2 | Taq DNA Polymerase | 2,000 U | $300.00 |
| E-2013-1 | Taq DNA Polymerase | 2,000 U | 10X Reaction Buffer, without 20 mM MgCl 2 | $415.38 |
| E-2013-3 | Taq DNA Polymerase | 2,000 U | $300.00 |
Literature / Support
- Manual_ Taq DNA Polymerase
- Manual_10X Reaction buffer for Taq DNA polymerase
- MSDS_10 mM dNTPs
- MSDS_20 mM MgCl 2
- MSDS_10X Reaction buffer without MgCl2 for Taq DNA polymerase
- MSDS_10X Reaction buffer for Taq DNA polymerase
- MSDS_Dilution buffer for Taq DNA polymerase
- MSDS_ Taq DNA polymerase
- ISO 9001 – certificate