The Autobio anti-HBs ELISA is designed for the qualitative determination of antibodies to hepatitis B surface antigen (anti-HBs) concentration in human serum or plasma specimens.
Anti-HBs titer can be determined to monitor the prognosis of patients recovering from the hepatitis B viral infection. It also can be used as an indicator of prior exposure to Hepatitis B viruses.
The anti-HBs ELISA is a solid-phase simultaneous immunoassay to detect antibodies against HBsAg. Microwells are coated with hepatitis B surface antigen (HBsAg). A serum specimen is added to the microwells together with horseradish peroxidase (HRP) Labeled HBsAg. After incubation, the complex of antigen-antibody-antigen (HRP-conjugated HBsAg, anti-HBs antibody and HBsAg on the wells) will be formed. Thus, the amount of HRP-HBsAg conjugate bound to the well is proportional to the concentration of anti-HBs in the specimen. The unbound enzyme conjugates will be washed away and then the chromogen and substrate solutions containing hydrogen peroxide is added to the wells. A blue color is developed in proportion to the amount of anti-HBs antibodies the specimens. The enzyme-substrate reaction is terminated with the addition of acid. The absorbance of controls and specimens is read in a microplate reader at the wavelength of 450 nm and 620nm.
