This assay is based on acid-fast staining technique, intended to apply chemical staining to acid-fast bacteria such as Mycobacterium tuberculosis, acting as an important aid in the diagnosis of Mycobacterium tuberculosis infections.
Globally, the TB disease is an enormous problem. TB is the world's most significant infectious disease from a single infection agent.
Hence, the early screening and detection of MTB infection is of great benefit to the control of this disease.
At the moment, the direct microscopic examination of stained sputum smear is still a commonly used method for the diagnosis of tuberculosis, due to its advantages such as fast, convenient and reliable results.